Effect of Honey/PVA Hydrogel Loaded by Erythromycin on Full-Thickness Skin Wound Healing in Rats; Stereological Study

  • Shabnam Movassaghi Department of Anatomical Sciences &Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Zahra Nadia Sharifi Department of Anatomical Sciences &Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
  • Mojtaba Koosha Department of Cellulose and Paper Technology, Faculty of New Technologies Engineering, Shahid Beheshti University, Zirab Campus, Savadkooh, Mazandaran, Iran
  • Mohammad Amin Abdollahifar Department of Biology and Anatomical Sciences, School of Medicine, Shahid Beheshti University of Medical Sciences, Tehran, Iran
  • Shahrzad Fathollahipour Department of Chemical and Biomolecular Engineering, University of Akron, Ohio 44325, USA
  • Javad Tavakoli Mechanical Engineering Biomechanics and Implants Research Group, The Medical Device Research Institute (MDRI), School of Computer Science Engineering and Mathematics, Sir Eric Neal Building, Flinders University, Adelaide, Australia
  • Shabnam Abdi Department of Anatomical Sciences &Cognitive Neuroscience, Faculty of Medicine, Tehran Medical Sciences, Islamic Azad University, Tehran, Iran
Keywords: Erythromycin, Honey, Polyvinyl Alcohol, Wound Healing


Background: Skin wounds are a significant public health risk, and treatment of wound remains a challenging clinical problem for medical teams and researchers.Materials and Methods: In the present study, we aimed to investigate the healing effects of honey/polyvinyl alcohol (PVA) hydrogel loaded with erythromycin  as wound dressing on skin wounds in rats, based on histological studies. In this study, 60 male Wistar rats, with a 1.5 ×1.5 cm2 diameter full-thickness wounds on the backs were divided into four groups: honey/PVA with the erythromycin hydrogel group, honey group, PVA group, and the control group, with no treatment. Skin biopsies were prepared at days 4, 7, and 14 for microscopic analyses. The stereological analysis, including the mean area of the wound, length of vessels, numerical density of fibroblast, macrophage, basal cell and volume of the epidermis, dermis, and fibrous tissue were performed. Result: Wounds area in the honey/PVA hydrogel with the erythromycin group were significantly (P<0.05) smaller than in the other group. The numerical density of fibroblast, macrophage, basal cell and volume of the epidermis in the honey/PVA hydrogel with the erythromycin group were significantly higher than other groups.Conclusion: According to our results, honey/PVA hydrogel with erythromycin may promote early wound healing and has a positive influence on fibroblast proliferation and re-epithelialization, and its administration is recommended after further validation of clinical data.[GMJ. 2019;8:e1362]


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How to Cite
Movassaghi, S., Sharifi, Z. N., Koosha, M., Abdollahifar, M. A., Fathollahipour, S., Tavakoli, J., & Abdi, S. (2019). Effect of Honey/PVA Hydrogel Loaded by Erythromycin on Full-Thickness Skin Wound Healing in Rats; Stereological Study. Galen Medical Journal, 8, e1362. https://doi.org/10.31661/gmj.v8i0.1362
Original Article