Effects of Carnitine with and without Glutamine Supplementation on Markers of Muscle Damage and Muscle Soreness among Football Players: A Randomized Controlled Clinical Trial
AbstractBackground: Exercise-induced muscle damage can affect exercise performance. The purpose of this study is to examine the effects of Carnitine and Glutamine supplementation on markers of muscle damage and muscle soreness after physical exertion on football players.Materials and Methods: Twenty eight healthy male football players aged 21.1±0.7 were recruited in a double blind, randomized, placebo-controlled clinical trial on 3 weeks of supplementation. Before supplementation protocol, each participant had to run on a treadmill for 30 minutes at 75% VO2max. Participants were randomly divided into 4 groups: L-Carnitine, L- Glutamine, L-Carnitine plus L- Glutamine and placebo. Blood samples were obtained pre-exercise and immediately after exercise. Muscle soreness was assessed on both occasions and two days after each exercise.Results: L-Carnitine and L-Glutamine supplementation for 21 days significantly decreased Creatine Kinase activity as a marker of muscle’s damage before (P=0.014) and after exercise (P=0.047), and muscle soreness two days after physical exertion (P=0.057). However, Lactate Dehydrogenase activity was affected by Carnitine supplementation after exercise.Conclusion: Chronic oral supplementation of Carnitine and Glutamine before exercise can reduce chemical markers of muscle tissue damage after exercise. In addition, these supplements may reduce muscle pain after exercise and optimize the processes of muscle tissue repair.[GMJ. 2014;3(4):207-15]
Lazarim FL, Antunes-neto JMF, Da Silva FOC, et al. The upper values of plasma creatine kinase of professional soccer players during the brazilian national championship. J Sci Med sport.2009; 12:85-90.
Clarkson PM, Hoffman EP, Zambraski E, et al. ACTN3 and MLCK genotype associations with exertional muscle damage. J Appl Physiol. 2005; 99:564-569.
Kraemer W, Spiering B, Volek J, et al. Recovery from a national collagiate athletic association division I football game: muscle damage and hormonal status. J Strength Cond Res. 2009; 23(1):2-10.
Marcora S, Bosio A. Effect of exercise-induced muscle damage on endurance running performance in humans. Scand J Med Sci Sports. 2007;17(6):662-669.
Aydogdu N, Atmaca G, Yalcin O, et al. Protective effects of L-carnitine on myoglobinuric acute renal failure in rats. ClinExpPharmacol Physiol. 2006;33:119-124.
Ho J-Y, Kraemer WJ, Volek JS, et al. L-carnitine l-tartrate supplementation favorably affects biochemical markers of recovery from physical exertion in middle-aged men and women. Metabolism. 2010;59:1190-1199.
Kraemer WJ, Volek JS, French DN, et al. The effects of L-carnitine L-tartrate supplementation on hormonal responses to resistance exercise and recovery. J Strength Cond Res. 2003;17:455-462.
Volek JS, kraemer WJ, rubin MR, et al. L-Carnitine L-tartrate supplementation favorably affects markers of recovery from exercise stress. Am J PhysiolEndocrinolMetab. 2002;282:474-82.
Newsholme P. Why is L-glutamine metabolism important to cells of the immune system in health, postinjury, surgery or infection? J Nutr. 2001;131(9Suppl):2515S-2523S.
Nosaka K, Sacco P, Mawatari K. Effects of amino acid supplementation on muscle soreness and damage. Int J Sport NutrExercMetab. 2006;16(6):620-635.
Seifi-Skishahr F, Siahkohian M, Nakhostin-Roohi B. Influence of aerobic exercise at high and moderate intensities on lipid peroxidation in untrained men. J Sports Med Phys Fitness. 2008;48(4):515-521.
Broad EM, Maughan RJ, Galloway SDR. Carbohydrate, Protein, and Fat Metabolism During Exercise After Oral Carnitine Supplementation in Humans. International Journal of Sport Nutrition and Exercise Metabolism. 2008;18:567-84.
Tartibian B, Azadpoor N, Abbasi A. Effects of two different type of treadmill running on human blood leukocyte populations and inflammatory indices in young untrained men. J Sports Med Phys Fitness. 2009;49(2):214-223.
Haub M, Potteiger K, Nau K, et al. Acute L-glutamine ingestion does not improve maximal effort exercise. J Sports Med Phys Fitness. 1998;38:240-244.
Vina J, Gimeno A, Sastre J, et al. Mechanism of free radical production in exhaustive exercise in humans and rats; role of xanthine oxidase and protection by allopurinol. IUBMB Life. 2000;49:539-544.
Giamberardino MA, Dragani L, Valente R, et al. Effects of prolonged L-carnitine administration on delayed muscle pain and CK release after eccentric effort. Int J Sports Med. 1996;17:320-324.
Savitha S, Tamilselvan J, Anusuyadevi M, et al. Oxidative stress on mitochondrial antioxidant defense system in the aging process: Role of dl-[alpha]-lipoic acid and l-carnitine. ClinicaChimicaActa. 2005;355:173-180.
Savitha S, Naveen B, Panneerselvam C. Carnitine and lipoate ameliorates lipofuscin accumulation and monoamine oxidase activity in aged rat heart. Eur J Pharmacol. 2007;574(1):61-65.
Coombes JS, McNaughton LS.Effects of branched-chain amino acid supplementation on serum creatine kinase and lactate dehydrogenase after prolonged exercise. J Sports Med Phys Fitness. 2000;40(3):240-246.
Sugino T, Aoyagi S, Shirai T, et al. Effects of citric acid and L-carnitine on physical fatigue. J ClinBiochemNutr. 2007;41:224-230.
Cruzat VF, Rogero MM, Tirapegui J. Effects of supplementation with free glutamine and the dipeptide alanyl glutamine on parameters of muscle damage and inflammation in rats submitted to prolonged exercise. Cell Biochemistry and Function. 2010;28(1):24-30.
Tajari SN, Rezaee M, Gheidi N. Assessment of the effect of L-glutamine supplementation on DOMS. Br J Sports Med. 2010;44(Suppl 1):i43.
Sachan D.S, Hongu N. Increases in VO2max and metabolic markers of fat oxidation by caffeine, carnitine, and choline supplementation in rats. J NutrBiochem. 2000;11(10):521-526.
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