Dysregulation of miR-577, miR-505-3p, miR-3682-3p and miR-4661 in breast cancer patients, differentially expression in ER-positive compared to ER-negative
AbstractIt is proved that the breast cancer is one of the most common cancers and the second leading cause of cancer-related death in women. Approximately 75 % of all breast cancers is ER-positive which is highly responsive to endocrine therapy. MicroRNAs (miRNAs) are short non-coding RNA that have a pivotal role in mammal cells by regulating gene expression. The expression of miRNAs is different in the various subtypes of breast cancer, and the expression profile of miRNAs can be informative in the classification of breast cancer. In this study, after extraction of total RNA and synthesis of cDNA expression of miR-577, -505-3p, -3682-3p and -4661-5p were investigated respectively in eighteen breast cancer samples of both ER+ and ER- types and eighteen normal adjacent tissues by Real-Time PCR. Downregulation of miR-577 and miR-505-3p was detected in breast cancer samples. Expression of miR-577 and miR-505-3p significantly decreased in ER+ subtype compared to that of ER- subtype. Data has showed upregulation of miR-3682-3p and miR-4661-5p in breast cancer tissue. The expression level of this miRNA was higher in ER- subtype than that of the ER+ subtype. ROC analysis demonstrated that both miR-577 and miR-505-3p have an acceptable diagnostic value in diagnosing breast cancer and differentiation of various types of breast cancer. MiR-3682-3p has had a relatively proper diagnostic value in diagnosing breast cancer but it does not have adequate diagnostic power to discriminate the different subtypes of breast cancer. The data obtained from the present study revealed that both miR-577 and miR-505-3p could be used as a biomarker in diagnosing different subtypes of breast cancer.
Alyami NM. MicroRNAs Role in Breast Cancer: Theranostic Application in Saudi Arabia. Front Oncol. 2021;11:717759.
Shiovitz S, Korde LA. Genetics of breast cancer: A topic in evolution. Ann Oncol. 2015;26(7):1291-9.
Moo TA, Sanford R, Dang C, Morrow M. Overview of Breast Cancer Therapy. PET Clin. 2018;13(3):339-54.
Hua H, Zhang H, Kong Q, Jiang Y. Mechanisms for estrogen receptor expression in human cancer. Exp Hematol Oncol. 2018;7:24.
Cizeron-Clairac G, Lallemand F, Vacher S, Lidereau R, Bieche I, Callens C. MiR-190b, the highest up-regulated miRNA in ERα-positive compared to ERα-negative breast tumors, a new biomarker in breast cancers? BMC Cancer. 2015;15(1):499.
Macfarlane LA, Murphy PR. MicroRNA: Biogenesis, Function and Role in Cancer. Curr Genomics. 2010;11(7):537-61.
Wahid F, Shehzad A, Khan T, Kim YY. MicroRNAs: synthesis, mechanism, function, and recent clinical trials. Biochim Biophys Acta. 2010;1803(11):1231-43.
Jansson MD, Lund AH. MicroRNA and cancer. Mol Oncol. 2012;6(6):590-610.
Khordadmehr M, Shahbazi R, Ezzati H, Jigari-Asl F, Sadreddini S, Baradaran B. Key microRNAs in the biology of breast cancer; emerging evidence in the last decade. J Cell Physiol. 2019;234(6):8316-26.
Dalmay T, Edwards DR. MicroRNAs and the hallmarks of cancer. Oncogene. 2006;25(46):6170-5.
Hwang HW, Mendell JT. MicroRNAs in cell proliferation, cell death, and tumorigenesis. Br J Cancer. 2006;94(6):776-80.
Van Der Auwera I, Limame R, Van Dam P, Vermeulen PB, Dirix LY, Van Laere SJ. Integrated miRNA and mRNA expression profiling of the inflammatory breast cancer subtype. Br J Cancer. 2010;103:532-41.
Lan H, Lu H, Wang X, Jin H. MicroRNAs as potential biomarkers in cancer: opportunities and challenges. Biomed Res Int. 2015;2015:125094.
Jang JY, Kim YS, Kang KN, Kim KH, Park YJ, Kim CW. Multiple microRNAs as biomarkers for early breast cancer diagnosis. Mol Clin Oncol. 2021;14(2):31.
Loh HY, Norman BP, Lai KS, Rahman NMANA, Alitheen NBM, Osman MA. The Regulatory Role of MicroRNAs in Breast Cancer. Int J Mol Sci. 2019;20(19):4940.
Denkiewicz M, Saha I, Rakshit S, Sarkar JP, Plewczynski D. Identification of Breast Cancer Subtype Specific MicroRNAs Using Survival Analysis to Find Their Role in Transcriptomic Regulation. Front Genet. 2019;10:1047.
Men L, Nie D, Nie H. MicroRNA-577 inhibits cell proliferation and invasion in non-small cell lung cancer by directly targeting homeobox A1. Mol Med Rep. 2019;19(3):1875-82.
Luo Y, Wu J, Wu Q, Li X, Wu J, Zhang J, et al. miR-577 Regulates TGF-β Induced Cancer Progression through a SDPR-Modulated Positive-Feedback Loop with ERK-NF-κB in Gastric Cancer. Mol Ther. 2019;27(6):1166-82.
Jiang H, Ju H, Zhang L, Lu H, Jie K. microRNA-577 suppresses tumor growth and enhances chemosensitivity in colorectal cancer. J Biochem Mol Toxicol. 2017;31(6):e21888.
Wang LY, Li B, Jiang HH, Zhuang LW, Liu Y. Inhibition effect of miR-577 on hepatocellular carcinoma cell growth via targeting β-catenin. Asian Pac J Trop Med. 2015;8(11):923-9.
Zhang XT, Dong SH, Zhang JY, Shan B. MicroRNA-577 promotes the sensitivity of chronic myeloid leukemia cells to imatinib by targeting NUP160. Eur Rev Med Pharmacol Sci. 2019;23(16):7008-15.
Tang H, Lv W, Sun W, Bi Q, Hao Y. MiR-505 inhibits cell growth and EMT by targeting MAP3K3 through the AKT-NFκB pathway in NSCLC cells. Int J Mol Med. 2019;43(3):1203-16.
Kapora E, Feng S, Liu W, Sakhautdinova I, Gao B, Tan W. MicroRNA-505-5p functions as a tumor suppressor by targeting cyclin-dependent kinase 5 in cervical cancer. Biosci Rep. 2019;39(7):20191221.
Tang Y, Wu B, Huang S, Peng X, Li X, Huang X, et al. Downregulation of miR-505-3p predicts poor bone metastasis-free survival in prostate cancer. Oncol Rep. 2019;41(1):57-66.
Tian L, Wang Z, Hao J, Zhang X. miR‐505 acts as a tumor suppressor in gastric cancer progression through targeting HMGB1. J Cell Biochem. 2019;120(5):8044-52.
Ren L, Yao Y, Wang Y, Wang S. MiR-505 suppressed the growth of hepatocellular carcinoma cells via targeting IGF-1R. Biosci Rep. 2019;39(7):20182442.
Rong Z, Rong Y, Li Y, Zhang L, Peng J, Zou B, et al. Development of a Novel Six-miRNA-Based Model to Predict Overall Survival Among Colon Adenocarcinoma Patients. Front Oncol. 2020;10:26.
Yao B, Niu Y, Li Y, Chen T, Wei X, Liu Q. High-matrix-stiffness induces promotion of hepatocellular carcinoma proliferation and suppression of apoptosis via miR-3682-3p-PHLDA1-FAS pathway. J Cancer. 2020;11(21):6188-203.
Li S, Hang L, Ma Y, Wu C. Distinctive microRNA expression in early stage nasopharyngeal carcinoma patients. J Cell Mol Med. 2016;20(12):2259-68.
Rong H, Liu D. Identification of differentially expressed miRNAs associated with thermal injury in epidermal stem cells based on RNA sequencing. Exp Ther Med. 2020;19(3):2218-28.
Cho HJ, Baek GO, Seo CW, Ahn HR, Sung S, Son JA, et al. Exosomal microRNA‐4661‐5p-based serum panel as a potential diagnostic biomarker for early‐stage hepatocellular carcinoma. Cancer Med. 2020;9(15):5459-72.
Li X, An Z, Li P, Liu H. A prognostic model for lung adenocarcinoma patient survival with a focus on four miRNAs. Oncol Lett. 2017;14(3):299-5.
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